Violet Red Bile Dextrose Agar

Violet Red Bile Dextrose Agar (VRBDA) is a highly effective culture medium specifically designed for the identification and enumeration of intestinal bacteria, with a focus on Enterobacteriaceae. This medium is crucial in food safety testing and clinical microbiology, allowing researchers to accurately assess bacterial counts in various samples.

Key Components:

• Peptic Digest of Animal Tissue (7.0 g/L): Provides essential nutrients for bacterial growth.
• Yeast (3.0 g/L): Enhances growth and metabolic activity.
• Sodium Chloride (5.0 g/L): Maintains osmotic balance and supports bacterial viability.
• Glucose (10.0 g/L): Serves as a primary carbon source, promoting robust growth.
• Bile (1.5 g/L): Selectively inhibits Gram-positive bacteria, allowing for the isolation of Gram-negative organisms.
• Crystal Violet (0.002 g/L): Further inhibits non-target organisms.
• Neutral Red (0.03 g/L): Acts as a pH indicator, aiding in the visualization of colonies.
• Agar (13.0 g/L): Provides the necessary solid support for colony formation.

With a final pH of 7.3 ± 0.2 at 25℃, VRBDA offers optimal conditions for cultivating target bacteria while suppressing the growth of contaminants. To prepare, simply suspend 39.5 g of the medium in 1 L of deionized or distilled water, boil for a maximum of two minutes, and cool to approximately 50℃ before pouring into sterile petri dishes. This straightforward preparation method eliminates the need for autoclaving, making it accessible for laboratories of all sizes.

Using VRBDA in your research ensures accurate intestinal bacteria identification, making it an indispensable tool for microbiologists dedicated to food safety and public health. Whether for quality control in food production or clinical diagnostics, Violet Red Bile Dextrose Agar stands out as a reliable choice for efficient bacterial enumeration and analysis.