Lysine Iron Agar (LIA)

Lysine Iron Agar (LIA), manufactured by BABIO, is a specialized microbiological medium designed for the differentiation of enteric bacteria. It allows for the identification of microorganisms based on their ability to decarboxylate or deaminate lysine and produce hydrogen sulfide. This versatile medium is ideal for distinguishing between various enteric bacilli in clinical and research settings, particularly for studies on lysine decarboxylase activity and hydrogen sulfide detection.

The formula includes peptic digest of animal tissue (5.0 g/L), dextrose (1.0 g/L), L-lysine hydrochloride (10.0 g/L), and sodium thiosulfate (0.04 g/L), with a final pH of 6.7 ± 0.2 at 25°C. Ferric ammonium citrate and bromocresol purple enable visual differentiation, as the medium changes color during microbial activity, and the presence of hydrogen sulfide leads to a blackening of the medium.

Lysine Iron Agar is critical in microbiological testing for its ability to differentiate enteric organisms based on their enzymatic activities, making it an essential tool in both food hygiene detection and clinical microbiology. It supports robust growth and facilitates precise microbial identification by monitoring bacterial reactions with lysine and sulfur compounds.

Specifications:

• Packing: 250g / 500g
• Formula: Peptic Digest of Animal Tissue (5.0g/L), Dextrose (1.0g/L), L-lysine Hydrochloride (10.0g/L), Sodium Thiosulfate (0.04g/L), Ferric Ammonium Citrate (0.5g/L)
• Storage: Store tightly sealed at 5-30°C
• Shelf Life: 3 years from manufacturing date

For accurate identification of enteric bacteria and hydrogen sulfide detection, trust BABIO’s Lysine Iron Agar. Learn more about our microbiological media on BABIO’s official website.