Feline Leukemia Antigen (FELV Ag) Test Kit

Feline Leukemia Antigen (FELV Ag) Test Kit is used for the detection of feline leukemia antigen in cat serum.

【testing principle】

Feline leukemia is a common non-traumatic fatal disease in cats. It is a malignant neoplastic infectious disease caused by feline leukemia virus and feline sarcoma virus. The main features are malignant lymphoid tumor, myelogenous leukemia, degenerative thymus atrophy and non-regenerative anemia, among which the most serious in cats is malignant lymphoid tumor. The susceptibility is high in young cats and decreases with age.

This kit uses double antibody sandwich immunochromatography. After the specimen was added to the sample area, the feline leukemia antigen contained in the specimen was bound to the coated antibody labeled by colloidal gold on the specimen pad to form an antigen-gold antibody conjugate, which migrated to the membrane under the action of capillary. When moved to the T-line position, the binding is captured by the trapping antibody on the T-line to form the trapping antibody-antigen-gold antibody conjugate, which presents a magenta T-line. The red band displayed by the quality control line (C line) is the standard to determine whether the chromatographic process is normal, and also serves as the internal control standard of the product.

【 Package Specifications and components 】

Note: swabs are complimentary separately according to package specifications.

【Self-contained appliance】

Timepiece

【Storage and expiration date】

The kit is stored at 2-30℃. Do not freeze. Valid for 24 months; After the kit is opened, the reagent should be used as soon as possible.

【Sample requirement】

1. Test sample: cat serum.

2. The sample should be tested on the same day; Samples that cannot be tested on the same day should be stored at 2-8 ℃, and samples that exceed 24 hours should be stored at -20 ℃.

【Inspection method】

1.  Before use, restore the kit to room temperature (15-30℃).

2.  Remove the reagent card from the aluminum foil bag and place it on a clean platform.

3.  Unscrew the top tube cap on the diluent tube cap containing the sample, invert the diluent tube, squeeze the tube wall, and add 3-5 drops of specimen mixture into the sample hole (S hole) of the reagent card.

4.  The results can be read in 10-15 minutes.  The result is invalid after 15 minutes.

【 Result interpretation 】

Positive: Both the quality control line (C line) and the test line (T line) appear

Negative: Only the quality control line (C line) is available

Invalid: Quality control line does not appear, take a new device to retest

【Precautions】  

1.   This product is only used for qualitative testing and does not indicate the virus level in the sample.

2.   Test results of this product are for reference only and should not be used as the sole basis for diagnosis and treatment, but should be made by a physician after evaluating all clinical and laboratory evidence.

3.   A negative result may occur if the viral antigen present in the sample is below the detection limit of the assay, or if the antigen detected at the disease stage at which the sample was collected is not present.

4.   The operation should be carried out in strict accordance with the instructions.   Do not use expired or damaged products.

5.   The test card should be used within 1 hour after opening;   If the ambient temperature is higher than 30 ° C or more humid, it should be used immediately.

6.   If the T line has just begun to show color, and then the line color gradually fades or even disappears, in this case, the sample should be diluted several times and tested until the T line color is stable.

7.   This product is a disposable product.   Do not reuse it.